Description
Aviva Systems Biology LMX1B ELISA Kit (Human) (OKEH02309) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human LMX1B antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated LMX1B and incubated. The LMX1B found in the sample or standards competes with the biotinylated LMX1B for limited binding sites on the immobilized anti-Human LMX1B antibody. Excess unbound biotinylated LMX1B and sample or standard LMX1B is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated LMX1B captured in the well and inversely proportional to the amount of LMX1B which was contained in the sample or standard